Cryo-electron microscopy (cryo-EM) has recently undergone a resolution revolution, placing it as a leading method in Structural Biology.
This course will cover the basics of how this technique is used to examine biological macromolecules and supramolecular complexes, as well as organelles and cells in three dimensions using modern electron microscopes and image reconstruction techniques.
Topics will range from electron optics, basic design of a TEM, sample preparation, image acquisition, 3-D reconstruction, and structural modeling for different modalities of cryo-EM.
The class is cross-listed as BIMM 162, BGGN 262, CHEM 165, and CHEM 265.
Instructor: Prof. Elizabeth Villa